Streptavidin coated Intelligent Bead SystemTM (IBS) Isolation of any biotinylated target
For research use only under the supervision of a technically qualified individual. Not for use in clinical diagnostic procedures.
1. Product Format
One bottle of Streptavidin A-BeadsTM contains a suspension of 10 mg/mL streptavidin coated magnetic particles.
Streptavidin A-BeadsTM are polydisperse 1.8µm paramagnetic particles covalently coupled with streptavidin. The paramagnetic particles remain in solution over extended periods of time. Minimal mixing is required for the fast and efficient separation of biotin-labelled molecules.
A 5'-biotin labeled oligo(dT)20 probe is hybridized with mRNA and immobilized on Streptavidin A-BeadsTM. After binding, the mRNA can be eluted or processed directly for RT-amplification.
Solid Phase Amplification
The 5'-biotin labeled primer bound to Streptavidin A-BeadsTM may be used for amplification reactions directly on the magnetic particle. One amplified strand may be eluted for further detection or detected directly by hybridization on the solid support.
The high affinity of the
streptavidin/biotin interaction (Kd=10-15) allows the rapid and efficient biomagnetic isolation of biotin
labelled target molecules.
Streptavidin A-BeadsTM can be successfully used in cycling applications for target
The Streptavidin A-BeadsTM capture and binding capacity is 10-20% better than any competitive Streptavidin magnetic particle commercially available.
6. Procedure for testing the reagent
Equilibrate the unopened bottle of
Streptavidin A-BeadsTM reagent at room temperature. Prepare a dilution series of biotinylated alkaline phosphatase in buffer. Add
magnetic particles to 1 mL of each dilution and incubate without mixing for 10 min. Wash 4 times with TPBS
using a MagnetOnTM. Resuspend in 1 mL of TPBS and add
5 µL of the suspension to a white microtiter plate. Add 100 µL of AP-reagent to the appropriate wells. Incubate for 30 min. at
room temperature and measure intensity.
Aureon Biosystems GmbH
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Tel: +43 1 740 40 350
Fax: +43 1 740 40 359