An Ultrasensitive Platephotometer and Luminometer
Mediators PhL is a versatile combination of dispensers, photometer and luminometer in a compact instrument for all photometric and luminometric applications. The soft- ware allows convenient access to raw data, kinetic data, quantitative results and qualitative interpretation
PhL is delivered including powerful software, only a computer with Windows
operating system or Mac PowerPC and Microsoft Excel program is required.
3. Product description
instrument is designed to measure all photometric and luminometric
applications. For future developments in the luminescence research field,
it is already equipped with an highpass-filter. Using Mediators PhL
Luciferase, ATP and Aequorin measurements can be extended to extreme low
limits of detection and a dynamic range of 7,5 decades. The CV for
precision and reproducibility is <0,3%. Due to the high sen- sitivity,
the instrument allows to reduce reagent costs. Mediators PhL is equipped
with two dispensers which can be used for adding lysis reagent and
substrate as well as auto- mating dual or even triple reportergen assays.
The dispensing parts are made of glass, ceramic and teflon, therefore all
reagents can be used in the instrument. Mediators PhL is an impressive
photometer - it has a measurement range of 0,001 - 4,500 OD. It has four
filters (405, 450, 570, 620nm) and can be upgraded with additional 9
filters. Mediators PhL is provided with sophisticated software -
Quantitative and qualitative evaluation is performed automatically. Many
different routine tests for Aequorin, ATP, Alkaline Phosphatase, Dual
Reportergen Assays, ß-Galactosidase, Luciferases, Peroxidase etc. or
combination of processing steps like in Apoptosis Assay, Aequorin/Bacteria,
luciferase/ß-galactosidase are started by pressing one button. The
software calculates qualitative, quantitative results and ratios like
luciferase/protein, luciferase /ß-galactosidase or Bact.Luciferase/Number
of bacteria auto- matically.
instrument is used in all fields of diagnostic and research. Examples are
measurement TSH with aequorin in an ELISA, Salmonella species with AP and
LumiPhos Plus in an Hybridi- zation assay, Firefly Luciferase as reportergen,
homogenous detection of amplification sequences with a modified
Acridiniumester, Apoptosis with ATP/ADP.
measurement geometry consists of a three color LED be- low the microplate to
be measured, the filter wheel with 16 positions directly above the plate
and the Photomultipliertube above the filter wheel.
Very high hardware stability is a special feature of the instru- ment. In case of problems an exchange instrument is delivered within 24 to 48 hours.
Use light standard of Lumigen to compare the sensitivity of instruments
Values for Mediators PhL:
100µL in C96 White Maxisorp Fluoronunc, 25°C, 10 sek. Integral
Aureon Biosystems GmbH
Simmeringer Hauptstr. 24
A-1110 Vienna / Austria
Tel: +43 1 740 40 350
Fax: +43 1 740 40 359