Nucleic acid based assay for the rapid detection of Salmonella species.
The assay is performed very similarly to a regular ELISA procedure. The detection and confirmation is done with luminescence labelled reagent rather than colorimetric. The sensitivity by light detection is about 100 fold more sensitive than the colorimetric detection in ELISA's. In addition the nucleic acid based hybridization yields the specificity required for immediate confrmation. The sample is read by the the 96 well Mediators PhL, an ultrasensitive luminometer in less than 2 minutes. No target amplification is necessary.
1. Product Desciption
A fluoresceine labelled hybridization probe which reacts with the Salmonella RNA after lysis of the bacteria. The probe is detected with an anti-fluoresceine antibody conjugated with alkaline phospatase.
2. Test Principle
Detection and confirmation of all Salmonella spp. directly from enriched samples treated with Salmonella A-Beads. Alternatively colonies on culture media can be picked. The bacterial RNA is released from the bacteria upon lysis and is captured by an immobilized DNA-oligonucleotide. The captured RNA is hybridized with a fluoresceine labelled detection probe. This probe is detected by an alkaline phosphatase conjugated anti-fluoresceine antibody. The action of alkaline phosphatase on the fluoresceine substrate causes decomposition of a chemiluminescent intermediate and the energy released is emmitted as light, which can be measured in the Mediators PhL luminometer.
The overall sensitivity and specificity is near 100% according to the described method.
Capture of bacteria
Lysis of bacteria
Aureon Biosystems GmbH
Simmeringer Hauptstr. 24
A-1110 Vienna / Austria
Tel: +43 1 740 40 350
Fax: +43 1 740 40 359